Journal: PLoS ONE
Article Title: Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells
doi: 10.1371/journal.pone.0194847
Figure Lengend Snippet: (A) Cells were cultured in an osteogenic medium for 7, 16, or 21 days to RANKL gene. RANKL genes expression was the highest at 16 days post osteoinduction as well as the osteogenic markers Osterix, Runx2, Collagen3A1, Osteopontin and BSP (Fig 6B-6F). Mesenchymal stem cells derived from human adipose tissue (hASCs). Osteoblasts cultured for 7 days with osteoinduction medium (Ost. 7 days). Osteoblasts cultured for 16 days with osteoinduction medium (Ost. 16 days). Osteoblasts cultured for 21 days with osteoinduction medium (Ost. 21 days). An ANOVA followed by Tukey's test was used to analyze the data. P <0.005.
Article Snippet: The expression levels of RANKL (Applied Biosystems TNFRSF11-Hs00243522_m1 assay), was performed using the TaqMan commercial qPCR kit (Invitrogen) according to the manufacturer's instructions.
Techniques: Cell Culture, Expressing, Derivative Assay